IBTK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88512

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKEICEVALTEKLTLKNAAMLLEFAAMYSAKQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSEFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDIFLKEEINMEQNHSETMFKKAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IBTK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]

Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]

Immunocytochemistry/Immunofluorescence: IBTK Antibody [NBP1-88512] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]

Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]

Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512] - Simple Western lane view shows a specific band for IBTK in 0.2 mg/ml of U-251 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512]

Simple Western: IBTK Antibody [NBP1-88512] - Electropherogram image(s) of corresponding Simple Western lane view. IBTK antibody was used at 1:20 dilution on U-251 lysate(s).
IBTK Antibody

Flow Cytometry: IBTK Antibody [NBP1-88512] -

Flow Cytometry: IBTK Antibody [NBP1-88512] - IBTK alpha RNA interference increases spontaneous & fludarabine–induced apoptosis in MEC-1 CLL cells MEC-1 cells were transduced with TurboGFP-shCTRL or TurboGFP-shIBTK alpha (Sigma-Aldrich), & after 5 days were left untreated (N.T.) a, b or 72 h-treated with Fludarabine at 25 µM c & d or 50 µM e, f. Then, cells were collected, stained with Annexin-V-APC & analysed by flow cytometry. Binding of Annexin-V-APC was measured for GFP-positive cells, as positive control of transduction. A representative experiment of three independent experiments is shown. Statistically significant differences were assessed using Mann–Whitney test (GraphPad Prism 6), as follows: a vs. bp = 0.0043; c vs. dp = 0.0002; e vs. fp = 0.0001 Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-017-0026-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IBTK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:20
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251, separated by Size, antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IBTK

IBTK is encoded by this gene binds to Bruton's tyrosine kinase (BTK) and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. [provided by RefSeq]

Alternate Names

Bruton agammaglobulinemia tyrosine kinase inhibitor, BTK-binding protein, BTKI, DKFZP564B116, IBtk, inhibitor of Bruton agammaglobulinemia tyrosine kinase, inhibitor of Bruton tyrosine kinase, inhibitor of Bruton's tyrosine kinase, inhibitor of Bruton's tyrosine kinase-alpha, inhibitor of Bruton's tyrosine kinase-beta, inhibitor of Bruton's tyrosine kinase-gamma, KIAA1417, MGC142256, MGC142258, RP1-93K22.1

Gene Symbol

IBTK

Additional IBTK Products

Product Documents for IBTK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IBTK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IBTK Antibody - BSA Free

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Protocols

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