IBTK Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-88512
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Scientific Data Images for IBTK Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: IBTK Antibody [NBP1-88512]
Immunocytochemistry/Immunofluorescence: IBTK Antibody [NBP1-88512] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512]
Immunohistochemistry-Paraffin: IBTK Antibody [NBP1-88512] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.Simple Western: IBTK Antibody [NBP1-88512]
Simple Western: IBTK Antibody [NBP1-88512] - Simple Western lane view shows a specific band for IBTK in 0.2 mg/ml of U-251 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Simple Western: IBTK Antibody [NBP1-88512]
Simple Western: IBTK Antibody [NBP1-88512] - Electropherogram image(s) of corresponding Simple Western lane view. IBTK antibody was used at 1:20 dilution on U-251 lysate(s).Flow Cytometry: IBTK Antibody [NBP1-88512] -
Flow Cytometry: IBTK Antibody [NBP1-88512] - IBTK alpha RNA interference increases spontaneous & fludarabine–induced apoptosis in MEC-1 CLL cells MEC-1 cells were transduced with TurboGFP-shCTRL or TurboGFP-shIBTK alpha (Sigma-Aldrich), & after 5 days were left untreated (N.T.) a, b or 72 h-treated with Fludarabine at 25 µM c & d or 50 µM e, f. Then, cells were collected, stained with Annexin-V-APC & analysed by flow cytometry. Binding of Annexin-V-APC was measured for GFP-positive cells, as positive control of transduction. A representative experiment of three independent experiments is shown. Statistically significant differences were assessed using Mann–Whitney test (GraphPad Prism 6), as follows: a vs. bp = 0.0043; c vs. dp = 0.0002; e vs. fp = 0.0001 Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-017-0026-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IBTK Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Simple Western
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251, separated by Size, antibody dilution of 1:20. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
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Background: IBTK
Alternate Names
Gene Symbol
Additional IBTK Products
Product Documents for IBTK Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for IBTK Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for IBTK Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars