ICAM-1/CD54 Antibody (ST0487)

Name: ICAM-1/CD54 Antibody (ST0487)
Brand: Novus Biologicals
SKU: NBP2-67518
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67518

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Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST0487 expressed in HEK293

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Product Specifications

Immunogen

Synthetic peptide within human ICAM-1/CD54 aa 30-70. (SwissProt: P05362 Human)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ICAM-1/CD54 Antibody (ST0487)

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Western Blot: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of ICAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: HUVEC cell lysate

Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/ Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Staining ICAM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human kidney tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human lung tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (ST0487) [NBP2-67518] - Analysis of paraffin-embedded human spleen tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.

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Applications for ICAM-1/CD54 Antibody (ST0487)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (ST0487)

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Product Specific Notices for ICAM-1/CD54 Antibody (ST0487)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.