ICAM-1/CD54 Antibody - BSA Free

Name: ICAM-1/CD54 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-88700
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-88700

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ICAM-1/CD54 Antibody - BSA Free

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88700 (A) shows similar protein distribution across tissues to independent antibody NBP1-88701 (B).

Western Blot: ICAM-1/CD54 Antibody [NBP1-88700]

Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung tissue.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lymph node shows moderate membranous positivity.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lung shows moderate membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: ICAM-1/CD54 Antibody - BSA Free [NBP1-88700] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for ICAM-1/CD54 Antibody - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody - BSA Free

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Product Specific Notices for ICAM-1/CD54 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.