IDH3A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16916

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IDH3A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH3A. The exact sequence is proprietary.

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IDH3A Antibody - BSA Free

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP2-16916]

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP2-16916]

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP2-16916] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
IDH3A Antibody

Immunohistochemistry: IDH3A Antibody [NBP2-16916] -

Immunohistochemistry: IDH3A Antibody [NBP2-16916] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using IDH3A antibody (NBP2-16916) at 1:300 dilution.
IDH3A Antibody

Western Blot: IDH3A Antibody [NBP2-16916] -

Western Blot: IDH3A Antibody [NBP2-16916] - Sample (30 ug of whole cell lysate)
A: whole zebrafish
B: zebrafish heart
10% SDS PAGE
NBP2-16916 diluted at 1:1000
IDH3A Antibody

Western Blot: IDH3A Antibody [NBP2-16916] -

Western Blot: IDH3A Antibody [NBP2-16916] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP2-16916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
IDH3A Antibody - BSA Free

Western Blot: IDH3A Antibody [NBP2-16916] -

IDH3A antibody detects IDH3A protein by Western blot analysis.
A. 50 ug rat brain lysate/extract
10 % SDS-PAGE
IDH3A antibody (NBP2-16916) dilution: 1:10000
IDH3A Antibody - BSA Free

Western Blot: IDH3A Antibody [NBP2-16916] -

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
NBP2-16916 diluted at 1:10000

Applications for IDH3A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IDH3A

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Alternate Names

EC 1.1.1, EC 1.1.1.41, H-IDH alpha, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitrate dehydrogenase 3 (NAD+) alpha, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH

Gene Symbol

IDH3A

UniProt

P50213 (Human)

Additional IDH3A Products

Product Documents for IDH3A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IDH3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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