IFIT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31164

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFIT2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFIT2 Antibody - BSA Free

Western Blot: IFIT2 Antibody [NBP1-31164]

Western Blot: IFIT2 Antibody [NBP1-31164]

IFIT2-Antibody-Western-Blot-NBP1-31164-img0006.jpg
Western Blot: IFIT2 Antibody [NBP1-31164]

Western Blot: IFIT2 Antibody [NBP1-31164]

Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
IFIT2 Antibody

Western Blot: IFIT2 Antibody [NBP1-31164] -

Western Blot: IFIT2 Antibody [NBP1-31164] - p115 & Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA & DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115 (A) or Sec16A (B) & non-targeting (NT) control shRNA for 24 h followed by puromycin selection (1.5 µg/ml) for 5 days. Cells were left untreated or stimulated with poly I:C (1 µg/ml), poly dA:dT (1 µg/ml) or SeV (200 HAU/ml) for 16 h. Whole-cell lysates were prepared & subjected to immunoblot analysis with indicated antibodies. One of two independent experiments with similar results is shown. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22792062), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
IFIT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP1-31164] -

IFIT2 antibody [N1], N-term detects IFIT2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term (NBP1-31164) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.
Blue: Hoechst 33342 staining.

Applications for IFIT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-31164 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIT2

Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Alternate Names

cig42, G10P2IFI-54K, GARG-39, IFI-54, IFI54IFIT-2, IFI-54K, Interferon, alpha-inducible protein (MW 54kD), Interferon-induced 54 kDa protein, interferon-induced protein 54, interferon-induced protein with tetratricopeptide repeats 2, ISG54, ISG-54 K, ISG-54K, P54

Gene Symbol

IFIT2

Additional IFIT2 Products

Product Documents for IFIT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IFIT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for IFIT2 Antibody - BSA Free

Customer Reviews for IFIT2 Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
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Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T, Hela, HEC-1-B, MRC-5 fibroblasts, whole cell extract following Senadi virus infection for 8-24h (200HAU/ml)
    Species: Human
    Verified Customer | Posted 07/17/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T
    Species: Human
    Verified Customer | Posted 07/16/2015

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