IFIT3 Antibody (OTI1G1)

Novus Biologicals | Catalog # NBP2-02148

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G1
View all IFIT3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFIT3 Antibody (OTI1G1)

Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3.
Flow Cytometry: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Flow Cytometry: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Flow Cytometry: IFIT3 Antibody (1G1) [NBP2-02148] - Analysis of Jurkat cells, using anti-IFIT3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Western Blot: IFIT3 Antibody (1G1) [NBP2-02148] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.
Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148]

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (1G1) [NBP2-02148] - Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody.
IFIT3 Antibody (OTI1G1)

IFIT3 Antibody (OTI1G1)[NBP2-02148]

IFIT3 Antibody (OTI1G1)[NBP2-02148]Simple Western� analysis of endogenous protein GBE1 from SH-SY5Y lysates (0.1mg/mL) using GBE1 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for IFIT3 Antibody (OTI1G1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIT3

IFIT3, also known as IFIT4, IFI60, and RIGG, has been identified as a key element in the IFN-alpha anti-viral pathway. Additionally, IFIT3 binding negatively regulates the apoptotic effects of ISG54. IFIT3 is also a key target of STAT1, which is important in regulating IFN responses. One study has shown that expression of RIGG in a human monocytoma line led to growth arrest at the G1/S transition.

Alternate Names

CIG-49, GARG-49, IFI60Retinoic acid-induced gene G protein, IFIT-3, IFIT-4, IFIT4IFI-60K, interferon-induced protein with tetratricopeptide repeats 3, Interferon-induced protein with tetratricopeptide repeats 4Interferon-induced 60 kDa protein, IRG2, ISG60, ISG-60, RIG-GCIG49

Entrez Gene IDs

3437 (Human)

Gene Symbol

IFIT3

Additional IFIT3 Products

Product Documents for IFIT3 Antibody (OTI1G1)

Certificate of Analysis

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Product Specific Notices for IFIT3 Antibody (OTI1G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IFIT3 Antibody (OTI1G1)

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Protocols

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