IFIT3 Antibody (OTI1G1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71006

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G1

Format

Azide and BSA Free
View all IFIT3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFIT3 Antibody (OTI1G1) - Azide and BSA Free

Western Blot: IFIT3 Antibody (OTI1G1)Azide and BSA Free [NBP2-71006]

Western Blot: IFIT3 Antibody (OTI1G1)Azide and BSA Free [NBP2-71006]

Western Blot: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.
Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Immunocytochemistry/ Immunofluorescence: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006] - Staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody.
Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3.
Western Blot: IFIT3 Antibody (OTI1G1)Azide and BSA Free [NBP2-71006]

Western Blot: IFIT3 Antibody (OTI1G1)Azide and BSA Free [NBP2-71006]

Western Blot: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006]

Flow Cytometry: IFIT3 Antibody (OTI1G1) - Azide and BSA Free [NBP2-71006] - Analysis of Jurkat cells, using anti-IFIT3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Applications for IFIT3 Antibody (OTI1G1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFIT3

IFIT3, also known as IFIT4, IFI60, and RIGG, has been identified as a key element in the IFN-alpha anti-viral pathway. Additionally, IFIT3 binding negatively regulates the apoptotic effects of ISG54. IFIT3 is also a key target of STAT1, which is important in regulating IFN responses. One study has shown that expression of RIGG in a human monocytoma line led to growth arrest at the G1/S transition.

Alternate Names

CIG-49, GARG-49, IFI60Retinoic acid-induced gene G protein, IFIT-3, IFIT-4, IFIT4IFI-60K, interferon-induced protein with tetratricopeptide repeats 3, Interferon-induced protein with tetratricopeptide repeats 4Interferon-induced 60 kDa protein, IRG2, ISG60, ISG-60, RIG-GCIG49

Gene Symbol

IFIT3

Additional IFIT3 Products

Product Documents for IFIT3 Antibody (OTI1G1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IFIT3 Antibody (OTI1G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IFIT3 Antibody (OTI1G1) - Azide and BSA Free

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Protocols

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FAQs

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