IFITM1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77171
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the center of human IFITM1. The immunogen is located within amino acids 40 - 90 of IFITM1. Amino Acid Squence: CAFAYSVKSRDRKMVGD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for IFITM1 Antibody - BSA Free
Western Blot: IFITM1 AntibodyBSA Free [NBP1-77171]
Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171]
Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-77171] - Jurkat cells with IFITM1 antibody at 20 ug/mL.Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] -
Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] - Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/mL.Western Blot: IFITM1 Antibody - BSA Free [NBP1-77171] -
ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: IFITM1 Antibody - BSA Free [NBP1-77171] -
ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for IFITM1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IFITM1
Long Name
Interferon-induced Transmembrane Protein 1
Alternate Names
CD225, IFI17, Leu-13
Gene Symbol
IFITM1
UniProt
Additional IFITM1 Products
Product Documents for IFITM1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IFITM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for IFITM1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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