IFITM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77171

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the center of human IFITM1. The immunogen is located within amino acids 40 - 90 of IFITM1. Amino Acid Squence: CAFAYSVKSRDRKMVGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFITM1 Antibody - BSA Free

Western Blot: IFITM1 AntibodyBSA Free [NBP1-77171]

Western Blot: IFITM1 AntibodyBSA Free [NBP1-77171]

Western Blot: IFITM1 Antibody [NBP1-77171] - 3T3 cell lysate with IFITM1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171]

Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171]

Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-77171] - Jurkat cells with IFITM1 antibody at 20 ug/mL.
IFITM1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] -

Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody - BSA Free [NBP1-77171] - Immunofluorescence of IFITM1 in Jurkat cells with IFITM1 antibody at 20 ug/mL.
IFITM1 Antibody - BSA Free

Western Blot: IFITM1 Antibody - BSA Free [NBP1-77171] -

ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
IFITM1 Antibody - BSA Free

Western Blot: IFITM1 Antibody - BSA Free [NBP1-77171] -

ADAR2 deficiency leads to upregulation of type 1 IFN response. (A) Basal expression of ISGs—RIG‐I and IFITM1 in Mero95 and RN5 WT, KD and rescue cells. Protein levels are represented relative to the respective WT. Protein size is represented in kDa. n = 3. One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. (B) Expression of ISGs—Rig‐I and Ifitm1 upon silencing of Mavs in RN5 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (C) Expression of ISGs – RIG‐I and IFITM1 upon silencing of STING in Mero95 ADAR2 KD cells. Protein levels are represented relative to siNT. Protein size is represented in kDa. n = 3. Paired t‐test. Error bars indicate SEM. (D) Representative images of senescence‐associated beta ‐Gal staining in Mero95 WT, KO and rescue cells (left). Quantification of beta ‐Gal positive cells (right). n = 3 (with at least 15 images from each group). One‐way ANOVA with post‐hoc Tukey's test. Error bars indicate SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36221913), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IFITM1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFITM1

IFITM1 (Interferon inducible transmembrane protein 1) is a member of the IFN-inducible transmembrane protein family. It is an essential mediator of interferon-gamma-induced antiproliferation and plays a role in the control of cell growth. IFITM1 is upregulated in several tumor types and may be useful as a tumor biomarker. Both mouse IFITM1 and IFITM3 are expressed on the cell surfaces of primordial germ cells in a developmentally-regulated manner. IFITM1 activity is required for primordial germ cell transit, and IFITM1 acts as a repulsive molecule by repelling non-IFITM1-expressing primordial germ cells from the mesoderm into the endoderm.

Long Name

Interferon-induced Transmembrane Protein 1

Alternate Names

CD225, IFI17, Leu-13

Gene Symbol

IFITM1

UniProt

Additional IFITM1 Products

Product Documents for IFITM1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IFITM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for IFITM1 Antibody - BSA Free

Customer Reviews for IFITM1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review IFITM1 Antibody - BSA Free and earn rewards!

Have you used IFITM1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...