Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Rat IFN-alpha ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-18572
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 ul)
Sensitivity
9.38pg/ml
Assay Range
15.63~1000pg/ml
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Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
No significant cross-reactivity or interference between Rat IFN-alpha and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 10%
Inter-Assay Precision (Precision between assays) CV% 10%
Recovery for Rat IFN-alpha ELISA Kit (Colorimetric)
Recovery
average = 98% for serum; average = 96% for EDTA plasma; average = 92% for cell culture media
Linearity
Scientific Data Images for Rat IFN-alpha ELISA Kit (Colorimetric)
ELISA: Rat IFN-alpha ELISA Kit (Colorimetric) [NBP3-18572] -
ELISA: Rat IFN-alpha ELISA Kit (Colorimetric) [NBP3-18572] - Standard Curve ReferencePreparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Rat IFN-alpha ELISA Kit (Colorimetric)
Certificate of Analysis
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Product Specific Notices for Rat IFN-alpha ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Rat IFN-alpha ELISA Kit (Colorimetric)
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Q: What is the level of endotoxin in the IFN-alpha products from PBL?
A: PBL states that the endotoxin level in their IFN-alpha products is <1EU/µg.
