IgE Antibody (7H2) - BSA Free

Novus Biologicals | Catalog # NBP3-11624

Novus Biologicals
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Key Product Details

Species Reactivity

Equine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H2

Format

BSA Free
View all IgE Primary Antibodies »

Product Specifications

Immunogen

Equine IgE

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for IgE Antibody (7H2) - BSA Free

ELISA: IgE Antibody (7H2) [NBP3-11624]

ELISA: IgE Antibody (7H2) [NBP3-11624]

ELISA: IgE Antibody (7H2) [NBP3-11624] - Purified Horse IgE detected in indirect ELISA with Mouse anti Horse IgE antibody, clone 7H2 followed by Rabbit F(ab')2 anti Mouse IgG:HRP.

Applications for IgE Antibody (7H2) - BSA Free

Application
Recommended Usage

ELISA

1:40 - 1:400
Application Notes
IHC-P: This antibody is demonstrated to work in formalin fixed tissue when using trypsin digestion pre-treatment of paraffin sections. Testing undertaken utilizing heat mediated antigen retrieval techniques with this antibody have not been successful. WB: Non-reducing conditions are recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgE

IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.

Long Name

Immunoglobulin E

Alternate Names

IgE, immunoglobulin heavy constant epsilon

Gene Symbol

IGHE

Additional IgE Products

Product Documents for IgE Antibody (7H2) - BSA Free

Certificate of Analysis

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Product Specific Notices for IgE Antibody (7H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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