IGF-II R/IGF2R Antibody

Novus Biologicals | Catalog # NB100-74387

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Plant, Primate

Cited:

Plant

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot, Tissue Culture Substratum

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IGF-II R/IGF2R Primary Antibodies »

Product Specifications

Immunogen

300 kDa cation-independent receptor purified from adult bovine liver tissue.

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26053072).

Marker

Late Endosome Marker

Specificity

CI-MPR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IGF-II R/IGF2R Antibody

Western Blot: IGF-II R/IGF2R Antibody [NB100-74387]

Western Blot: IGF-II R/IGF2R Antibody [NB100-74387]

Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody [NB100-74387]

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).

Applications for IGF-II R/IGF2R Antibody

Application
Recommended Usage

Dot Blot

1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Western Blot

1:2000 - 1:3000
Application Notes
In WB: Detects a 300 kDa protein representing Cl-MPR. May be useful in IP.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IGF-II R/IGF2R

The Cation independent Mannose 6 Phosphate Receptor transports phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose 6 phosphate receptors in the Golgi apparatus and the resulting receptor ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Entrez Gene IDs

3482 (Human); 16004 (Mouse); 281849 (Bovine)

Gene Symbol

IGF2R

UniProt

P08169 (Bovine)

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IGF-II R/IGF2R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IGF-II R/IGF2R Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IGF-II R/IGF2R Antibody

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  • Q: What is your modified RIPA buffer recipe?

    A: 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS

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