IgG Antibody (IG266)

Novus Biologicals | Catalog # NBP2-34250

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Cited:

Western Blot, ELISA, RNA immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # IG266
View all IgG Primary Antibodies »

Product Specifications

Immunogen

Purified human IgG

Localization

Cytoplasm, Cell Surface and Secreted

Marker

B-Cell Marker

Specificity

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34648)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for IgG Antibody (IG266)

Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) [NBP2-34250]

Immunocytochemistry/ Immunofluorescence: IgG Antibody (IG266) [NBP2-34250]

Immunocytochemistry/Immunofluorescence: IgG Antibody (IG266) [NBP2-34250] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250]

Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250]

Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).
IgG Antibody (IG266)

Protein Array: IgG Antibody (IG266) [NBP2-34250] -

Protein Array: IgG Antibody (IG266) [NBP2-34250] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for IgG Antibody (IG266)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34250 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

UniProt

P01857, P01859 (Human), P01861 (Human), P01860 (Human)

Additional IgG Products

Product Documents for IgG Antibody (IG266)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IgG Antibody (IG266)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IgG Antibody (IG266)

Customer Reviews for IgG Antibody (IG266) (1)

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Customer Images

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  • IgG Antibody (IG266)
    Name: Natalie Erdmann
    Application: Immunocytochemistry
    Sample Tested: LP-1 (multiple myeloma) and H929 (multiple myeloma)
    Species: Human
    Verified Customer | Posted 05/28/2019
    Immunocytochemistry image of LP-1 and H929 staining with NBP2-34250 antibody.
    1. Cells immersion-fixed in 2%PFA 10 minutes, wash PBS X 3 2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3 3. Block 10% donkey serum 1 hour 4. 1/200 NBP2-34250 O/N @4oC; wash PBS X 3 5. 1/1000 donkey F(ab’)2 Alexa Fluor 647 anti-mouse IgG (H+L) 1 hour @room temp (Jackson #715-606-150); wash PBS X 3 6. Olympus/BIOVIEW Cy5 filter 600ms exposure
    IgG Antibody (IG266) NBP2-34250

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Protocols

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