Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
Novus Biologicals | Catalog # NBP1-51564
![Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]](https://resources.rndsystems.com/images/products/Mouse-anti-Human-IgG-Fc-fragment-Secondary-Antibody-4A10-Western-Blot-NBP1-51564-img0006.jpg "Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4A10
Format
BSA Free
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Product Specifications
Immunogen
Purified fusion protein with IgG Fc tag.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]
Western Blot: Mouse anti-Human IgG Fc Secondary Antibody (4A10) [NBP1-51564] - Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag.![Immunocytochemistry/ Immunofluorescence: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]](https://resources.rndsystems.com/images/products/Mouse-anti-Human-IgG-Fc-fragment-Secondary-Antibody-4A10-Immunocytochemistry-Immunofluorescence-NBP1-51564-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]")
Immunocytochemistry/ Immunofluorescence: Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free) [NBP1-51564]
Immunocytochemistry/Immunofluorescence: Mouse anti-Human IgG Fc Secondary Antibody (4A10) [NBP1-51564] - Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using human IgGFc mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Applications for Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse anti-Human IgG Fc Secondary Antibody (4A10) (BSA Free)
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Q: Hi, do you know the concentration of NBP1-51564?
A: Unfortunately this antibody is unpurified provided in ascitic fluid, and hence the concentration is unquantifiable.
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