Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat.

Specificity

No reaction was observed against Mouse IgG F(ab) This antibody will react with heavy chains of Mouse IgG. Minimal reactivity is expected against other Mouse immunoglobulins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum

Scientific Data Images for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488]

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] [NBP1-72872] - This image shows anti-histone detection using a DyLight(TM) 488 conjugate (green). Anti-Tubulin was detected using a DyLight(TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1
Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] [NBP1-72872] - Analysis of Vero cells. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature. Image from verified customer review.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] [NBP1-72872] - Comparison of fluorescence absorption and emission spectra for Dylight 488(TM) and Alexa Fluor 488 (TM) in PBS, pH 7.2.

Applications for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

1:20000

Immunocytochemistry/ Immunofluorescence

1:5000

Western Blot

1:10000
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Reviewed Applications

Read 1 review rated 5 using NBP1-72872 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488]

Customer Reviews for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Vero cell
    Species: Other
    Verified Customer | Posted 11/12/2014
    kidney cell
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 488] NBP1-72872

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Protocols

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FAQs

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