Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunomicroscopy, Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Purified Mouse IgG Fc

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum proteins, light chains on all mouse immunoglobulins, mouse IgG, F(ab)'2 fragment

Specificity

Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.

Applications for Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:200-1:5000

Immunohistochemistry

1:500-1:5000

Immunomicroscopy

1:20-1:2000

Western Blot

1:200-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative

0.1% ProClin 150

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

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  • Q: I am going to use mouse antibody IP from Hela cells, and then to perform western blot. The first antibody is mouse. My protein size is same as IgG light chain. I need anti-mouse heavy chain specific HRP to avoid the light chain. Could you tell me which reagent is better? Is that NBP1-75179 can avoid IgG light chain?

    A: I have pulled up information on the antibody NBP1-75179 and believe this would be a great choice for you to use in your particular experiment. The lab has verified that it detects the heavy and not light chain and we would back this information that is indicated on the datasheet with our 100% guarantee.

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