Key Product Details

Species Reactivity

Goat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Donkey IgG

Format

Pre-adsorbed
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

This Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was developed against purified goat IgG (H&L).

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin goat serum immunoglobulins and IgG from human, mouse, rabbit, or rat

Specificity

Based on IEP, this Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) heavy gamma chains on goat IgG and light chains on all goat immunoglobulins. This antibody has been pre-adsorbed against human, mouse, rabbit or rat IgG

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Description

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Store lyophilized material at 2-8C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20C as a liquid.

Scientific Data Images for Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) [NBP1-74822] - 50 ug of mouse lung lysate per lane. Primary antibody: Goat anti-mouse JAM-C antibody. Secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) at 1:2500 in 5% milk/PBST. Image from verified customer review.
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) [NBP1-74822] - Lysates of mouse embryo tissue loaded at the indicated concentrations. PVDF membrane was probed with 0.2 ug/mL goat anti-P-cadherin polyclonal antibody (AF761) followed by a 1:5000 Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) (NBP1-74822).

Applications for Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

Application
Recommended Usage

ELISA

1:200-1:5000

Immunohistochemistry

1:500-1:5000

Western Blot

1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-74822 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Reconstitution

Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Formulation

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Format

Pre-adsorbed

Preservative

0.1% ProClin 150

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) (2)

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Customer Images

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  • Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)
    Name: James Xiao
    Application: Western Blot
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 04/16/2018
    50 ug of mouse lung lysate was used per lane. Primary antibody: Goat anti-mouse JAM-C antibody; Secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) @ 1:2500 in 5% milk/PBST.
    Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) NBP1-74822
  • Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human testis
    Species: Human
    Verified Customer | Posted 10/09/2017
    Primary antbibody: Human SOX9 Antibody (AF3075-SP) Secondary: Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) NBP1-74822 diluted 1/100
    Donkey anti-Goat IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) NBP1-74822

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