IgG Heavy Chain Antibody (RM116) [Biotin]

Novus Biologicals | Catalog # NBP2-62019B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM116 expressed in HEK293
View all IgG Heavy Chain Primary Antibodies »

Product Specifications

Immunogen

Human IgG

Specificity

This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including gamma 1, gamma 2, gamma 3, and gamma 4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG Heavy Chain Antibody (RM116) [Biotin]

IgG Heavy Chain Antibody (RM116) [Biotin]

ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] -

ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] - A titer ELISA using NBP2-62019B. The plate was coated with different amounts of human IgG1. A serial dilution of NBP2-62019B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG Heavy Chain Antibody (RM116) [Biotin]

ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] -

ELISA: IgG Heavy Chain Antibody (RM116) [Biotin] [NBP2-62019B] - ELISA of human immunoglobulins shows NBP2-62019B reacted to the gamma1, gamma2, gamma3, gamma4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of NBP2-62019B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG Heavy Chain Antibody (RM116) [Biotin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG Heavy Chain

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Heavy Chain Products

Product Documents for IgG Heavy Chain Antibody (RM116) [Biotin]

Certificate of Analysis

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Product Specific Notices for IgG Heavy Chain Antibody (RM116) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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