Key Product Details

Species Reactivity

Monkey

Applications

Immunohistochemistry, Western Blot, ELISA, SDS-Page

Label

Unconjugated

Antibody Source

Monkey IgG

Format

Purified
View all IgG Isotype Controls »

Product Specifications

Host

Monkey

Isotype

IgG

Description

This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey IgG and anti-Monkey Serum.

Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Monkey IgG Isotype Control

Western Blot: Monkey IgG Isotype Control [NBP1-97047]

Western Blot: Monkey IgG Isotype Control [NBP1-97047]

Western Blot: Monkey IgG Isotype Control [NBP1-97047] - SDS-PAGE of Monkey IgG Whole Molecule. Lane 1: Non-Reduced Monkey IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker. Lane 3: Reduced Monkey IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher due to large molecular weight.

Applications for Monkey IgG Isotype Control

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 500 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Monkey IgG Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Monkey IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Monkey IgG Isotype Control

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Monkey IgG Isotype Control

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  • Q: What is the species of monkey used to generate this IgG (old world, new world?)

    A: This product was prepared from a mixture of old world monkey species, including Cyno, Rhesus, etc. We can also make it from one species through custom order. 

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