Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Control

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # P3.6.2.8.1
View all IgG1 Kappa Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control.

Scientific Data Images for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] [NBP1-43319AF647]

Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] [NBP1-43319AF647]

Flow Cytometry: Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [Alexa Fluor (R) 647] [NBP1-43319AF647] - Mouse IgG1 isotype control was used as a negative control. Image from verified customer review.

Applications for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Tested for Flow analysis on normal human peripheral blood cells and mouse spleen cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-43319AF647 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50 mM Sodium Borate.

Preservative

0.05% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Product Documents for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Customer Reviews for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] (1)

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Customer Images

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human PBMC
    Species: Human
    Verified Customer | Posted 12/08/2014
    human PBMC
    Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647] NBP1-43319AF647

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Protocols

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FAQs for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Alexa Fluor® 647]

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  • Q: What is the concentration of NBP1-43319AF647?

    A: All of our directly conjugated antibodies are made to order and will have the final concentration measured at the completion of this process and will be listed on the product label. This is why our concentrations are not labeled on our datasheet. For item NBP1-43319AF647, the expected concentration range for the final product should be between 0.4-0.5mg/ml; with a total volume of 1.0ml.

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