Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin]
Novus Biologicals | Catalog # NBP1-43319APC
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] [NBP1-43319APC]](https://resources.rndsystems.com/images/products/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Allophycocyanin-Flow-Cytometry-NBP1-43319APC-img0001.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] [NBP1-43319APC]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Control
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # P3.6.2.8.1
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Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This Mouse IgG1 Kappa immunoglobulin is useful as an isotype control.
Scientific Data Images for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin]
Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] [NBP1-43319APC]
Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] [NBP1-43319APC] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.Applications for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin]
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Tested for Flow analysis on normal human peripheral blood cells and mouse spleen cells. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Product Documents for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Customer Reviews for Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] (1)
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Customer Images
![Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] NBP1-43319APC](https://resources.rndsystems.com/images/reviews/review_nbp1-43319apc_42866_0_0.jpg)
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Verified Customer | Posted 08/23/2018Analysis of Involucrin signal in human keratinocytes. Red : anti-IVL antibody BLACK : Isotypic control NBP1-43319APC at 1/100used at 1/100e in washing buffer, after permeabilization with cytofix/cytoperm kit (BD) on human keratinocytes
![Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [Allophycocyanin] NBP1-43319APC](data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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