IgG3 Antibody (RM119) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25976

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM119 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Human IgG3

Specificity

This antibody reacts to the heavy chain of human IgG3, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG3 Antibody (RM119) - Azide and BSA Free

IgG3 Antibody (RM119) - Azide and BSA Free

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] -

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] - ELISA of human immunoglobulins shows RM119 only reacts to human IgG3. No cross reactivity with other human immunoglobulins, mouse, rat, or goat IgG.
IgG3 Antibody (RM119) - Azide and BSA Free

Immunohistochemistry-Paraffin: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] -

Immunohistochemistry-Paraffin: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] - Immunohistochemistry of Human Lymphoid Tissue using Clone RM119.
IgG3 Antibody (RM119) - Azide and BSA Free

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] -

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] - ELISA of human immunoglobulins shows RM119 only reacts to human IgG3. No cross reactivity with other human immunoglobulins, mouse, rat, or goat IgG.
IgG3 Antibody (RM119) - Azide and BSA Free

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] -

ELISA: IgG3 Antibody (RM119) - Azide and BSA Free [NBP3-25976] - A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody.

Applications for IgG3 Antibody (RM119) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5ug/ml - 2ug/ml

Immunohistochemistry

0.5ug/ml - 2ug/ml

Immunohistochemistry-Paraffin

0.5ug/ml - 2ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G3

Alternate Names

DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809

Gene Symbol

IGHG3

Additional IgG3 Products

Product Documents for IgG3 Antibody (RM119) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IgG3 Antibody (RM119) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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