Rabbit anti-Mouse IgM (H+L) Secondary Antibody

Novus Biologicals | Catalog # NBP1-73946

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Mouse IgM whole molecule

Specificity

No reaction was observed against Mouse IgG F(c). Some light chain cross reactivity may occur against Mouse IgG.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgM

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Rabbit anti-Mouse IgM (H+L) Secondary Antibody

Application
Recommended Usage

ELISA

1:20000 - 1:100000

Immunohistochemistry

1:1000 - 1:5000

Western Blot

1:2000 - 1:10000
Application Notes
This product has been assayed against Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgM antibody is suitable for immunoassays including western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgM (H+L) Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgM (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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