Key Product Details

Species Reactivity

Monkey

Applications

Immunohistochemistry, Western Blot, ELISA

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Monkey IgM whole molecule

Specificity

No reaction was observed against other Monkey heavy or light chain proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum

Scientific Data Images for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody [HRP]. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block for 30 min at RT.Observed size: 72 kDa for Rabbit IgG. Other band(s): none.
N/A: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726]
Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Western Blot of Peroxidase conjugated Goat anti-Monkey IgM mu antibody. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Observed size: 72 kDa for Rabbit IgG. Other band(s): none.

Applications for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:65000 - 1:160000

Immunohistochemistry

1:500 -1:2500

Western Blot

1:1000 - 1:5000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can this antibody be used with samples from the Rhesus monkey or cross re-activity with Rhesus monkey ?

    A: This product is generally specific for Monkey IgM's which includes mostly Old World monkeys, like: Macaque, Cynomologus, Rhesus, Baboon, Chimpanzee, Great Ape and any other African species. Reactivity to New World primates has not yet been determined.

  • Q: Will this antibody cross react with Human IgM?

    A: Although it has not been specifically tested for human IgM, this antibody is predicted to cross react with human IgM. 

  • Q: Can this antibody be used with samples from the Rhesus monkey or cross re-activity with Rhesus monkey ?

    A: This product is generally specific for Monkey IgM's which includes mostly Old World monkeys, like: Macaque, Cynomologus, Rhesus, Baboon, Chimpanzee, Great Ape and any other African species. Reactivity to New World primates has not yet been determined.

  • Q: Will this antibody cross react with Human IgM?

    A: Although it has not been specifically tested for human IgM, this antibody is predicted to cross react with human IgM. 

Showing  1 - 2 of 2 FAQs Showing All
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