Ikaros/IKZF1 Antibody (S08-5E4)
Novus Biologicals | Catalog # NBP3-19660
Recombinant Monoclonal Antibody
![Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]](https://resources.rndsystems.com/images/products/Ikaros-IKZF1-Antibody-S08-5E4-Western-Blot-NBP3-19660-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S08-5E4 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human Ikaros/IKZF1 (Uniprot # Q13422)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ikaros/IKZF1 Antibody (S08-5E4)
Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]
Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660] - Western blot detection of Ikaros/IKZF1 in CHO-K1, Jurkat cell lysates using NBP3-19660 (1:500 diluted). Predicted band size: 58kDa. Observed band size: 50-70kDa.![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]](https://resources.rndsystems.com/images/products/Ikaros-IKZF1-Antibody-S08-5E4-Immunohistochemistry-Paraffin-NBP3-19660-img0001.jpg "Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]")
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660]
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660] - Immunohistochemistry of Ikaros/IKZF1 in paraffin-embedded Human tonsil using NBP3-19660 at dilution 1/50Applications for Ikaros/IKZF1 Antibody (S08-5E4)
Application
Recommended Usage
Immunohistochemistry
1/200
Western Blot
1/2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ikaros
Long Name
DNA-binding protein Ikaros
Alternate Names
IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1
Gene Symbol
IKZF1
Additional Ikaros Products
Product Documents for Ikaros/IKZF1 Antibody (S08-5E4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ikaros/IKZF1 Antibody (S08-5E4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
