Ikaros/IKZF1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38242
![Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242]](https://resources.rndsystems.com/images/products/Ikaros-IKZF1-Antibody-Western-Blot-NBP2-38242-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Applications
Simple Western, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DADEGQDMSQVSGKESPPVSDTPDEGDEPMPIPEDLSTTSGGQQSSKSDRVVASNVKVETQSDEENGRACEMNGEECAEDLRMLDASGEKMNGSH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ikaros/IKZF1 Antibody - BSA Free
Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242]
Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Chromatin Immunoprecipitation ChIP: Ikaros/IKZF1 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-Ikaros/IKZF1 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for Ikaros/IKZF1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Simple Western
1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: RPMI 8266 cell line at 1.0 mg/ml as sample; separated by size; antibody dilution of 1:50; observed molecular weight was 35-77 kDa; matrix was 12-230 kDa; detected by Chemiluminescence.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ikaros
Long Name
DNA-binding protein Ikaros
Alternate Names
IK1, IKZF1, LyF-1, LYF1, PRO0758, ZNFN1A1
Gene Symbol
IKZF1
UniProt
Additional Ikaros Products
Product Documents for Ikaros/IKZF1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ikaros/IKZF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Associated Pathways
