Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

IL-10 Antibody (2050B) [PE]

Novus Biologicals | Catalog # FAB9210P

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Neutralization, Intracellular Staining by Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG_2B Clone # 2050B

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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Met1-Asn178
Accession # P22301

Specificity

Detects human IL-10 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG_2B

Scientific Data Images for IL-10 Antibody (2050B) [PE]

IL-10 Antibody (2050B) [PE] [FAB9210P] -

IL-10 Antibody (2050B) [PE] [FAB9210P] - Vial of PE conjugated antibody. PE has two excitation maxima, 498 nm excited by the Blue laser (488 nm) and 565 nm excited by the Yellow-Green laser (561 nm). Both result in emission at 578 nm.

Applications for IL-10 Antibody (2050B) [PE]

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for IL-10 Antibody (2050B) [PE]

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Product Specific Notices for IL-10 Antibody (2050B) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.