Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

IL-10 Antibody Pair [HRP]

Novus Biologicals | Catalog # NBP2-79291

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

14.06-900 pg/ml (example only; lot dependent)

Sensitivity

14.06 pg/ml (example only; lot dependent)

Reactivity

Human

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Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human IL-10.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for IL-10 Antibody Pair [HRP]

Sandwich ELISA: IL-10 Antibody Pair [HRP] [NBP2-79291]

Kit Contents for IL-10 Antibody Pair [HRP]

Mouse Monoclonal Capture Antibody
Rabbit Polyclonal Detection Antibody (HRP-conjugated)
Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for IL-10 Antibody Pair [HRP]

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Download Manual - IL-10 Antibody Pair [HRP]

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Product Specific Notices for IL-10 Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.