Monkey IL-10 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-92708

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (baboon, cynomolgus) (50 uL)

Sensitivity

1.3 U/mL

Assay Range

3.9 - 250 U/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Monkey

Specificity

The interference of circulating factors of the immune systemwas evaluated by spiking these proteins at physiologically relevant concentrations into a monkey IL-10 positive serum. There was no crossreactivity detected.

Description

The monkey IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-10.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.0%

Inter-Assay Precision (Precision between assays) CV% 10%

Scientific Data Images for Monkey IL-10 ELISA Kit (Colorimetric)

ELISA: Monkey IL-10 ELISA Kit (Colorimetric) [NBP1-92708]

Kit Contents for Monkey IL-10 ELISA Kit (Colorimetric)

  • Adhesive Plate Covers
  • Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey IL-10, Biotin-Conjugate (anti-monkey IL-10 monoclonal antibody), Sample Diluent and Streptavidin-HRP, lyophilized
  • Aluminium pouch(es) with a monkey IL-10 Standard curve (coloured)
  • Sample Diluent (Use when an external predilution of the samples is needed)
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-10

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for Monkey IL-10 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Monkey IL-10 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Monkey IL-10 ELISA Kit (Colorimetric)

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    • A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.
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