IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09027

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Cited:

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 14.1

Format

Azide and BSA Free
View all IL-16 Primary Antibodies »

Product Specifications

Immunogen

The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

Flow Cytometry: IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free [NBP3-09027]

Flow Cytometry: IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free [NBP3-09027]

Flow Cytometry: IL-16 Antibody (14.1) - Chimeric [NBP3-09027] - Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (NBP3-09027, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with NBP3-09027. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Applications for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF- alpha, and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-16

Interleukin-16 (IL-16, also known as lymphocyte chemoattractant factor (LCF)) is a CD8+ T cell-derived cytokine. It acts as a chemoattractant for CD4+ T-cells, monocytes and eosinophils. IL-16 has also been shown to suppress HIV-1 replication in vitro. It is expressed as a precursor molecule (pro-IL-16) in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The active form is assumed to be a proteolytic cleavage product of pro-IL-16 existing, under physiological conditions, predominantly as a noncovalently linked multimer. The sequence and structure of IL-16 is conserved across species, human and murine IL-16 show significant cross-species reactivity.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

Additional IL-16 Products

Product Documents for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-16 Antibody (14.1) - Chimeric - Azide and BSA Free

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Protocols

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FAQs

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