IL-16 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-21240

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IL-16 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRARSFPLTRSQSCETKLLDEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNLSELREYTEGLTEAKEDDDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit IL-16 Antibody - BSA Free (NBP3-21240) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-16 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: IL-16 Antibody [NBP3-21240] -

Immunocytochemistry/Immunofluorescence: IL-16 Antibody [NBP3-21240] -

Staining of human cell line U-2 OS shows positivity in nucleus, plasma membrane & cytoplasm.
Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Analysis in human lymph node and cerebral cortex tissues using Anti-IL16 antibody. Corresponding IL16 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Immunohistochemistry-Paraffin: IL-16 Antibody [NBP3-21240] -

Staining of human lymph node shows high expression.

Applications for IL-16 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-16

Interleukin-16 (IL-16, also known as lymphocyte chemoattractant factor (LCF)) is a CD8+ T cell-derived cytokine. It acts as a chemoattractant for CD4+ T-cells, monocytes and eosinophils. IL-16 has also been shown to suppress HIV-1 replication in vitro. It is expressed as a precursor molecule (pro-IL-16) in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. The active form is assumed to be a proteolytic cleavage product of pro-IL-16 existing, under physiological conditions, predominantly as a noncovalently linked multimer. The sequence and structure of IL-16 is conserved across species, human and murine IL-16 show significant cross-species reactivity.

Long Name

Interleukin 16

Alternate Names

IL16, LCF

Gene Symbol

IL16

Additional IL-16 Products

Product Documents for IL-16 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IL-16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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