Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot, ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

This purified IL-17/IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL-17/IL-17A protein. (Uniprot: Q61453)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26356122).

Specificity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IL-17/IL-17A Antibody - Azide and BSA Free

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.
Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.
Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.
Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A AntibodyAzide and BSA Free [NBP1-42772]

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.
IL-17/IL-17A Antibody - Azide and BSA Free

IL-17/IL-17A Antibody - Azide and BSA Free

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG
IL-17/IL-17A Antibody - Azide and BSA Free

IL-17/IL-17A Antibody - Azide and BSA Free

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG
IL-17/IL-17A Antibody - Azide and BSA Free

Western Blot: IL-17/IL-17A Antibody - Azide and BSA Free [NBP1-42772] -

∆pep27 immunization repressed proinflammatory cytokines expression via Tregs upregulation to enhance barrier permeability. (A) Heat map analysis revealed inhibition of inflammation-related genes, antioxidant genes, and repression of the caspase-14 by ∆pep27 in colon. (B–F) Caspase-14 transcription (B), Treg transcription factor and anti-inflammatory mediators (C–E) in DIC were determined by qPCR. (F) Levels of various proteins were confirmed by western blot analysis using beta -actin as an internal control. Band intensity was quantified using ImageJ 2.1.4.6 software (National Institutes of Health, Bethesda, MD, USA). (G,H) Epithelial barrier disruption was determined by tight junction gene (G) and Evans blue dye assay (H). (I–O) Protein expression was quantified by band density and analyzed by ImageJ 2.1.4.6 software (National Institutes of Health, Bethesda, MD, USA). Statistically significant differences were defined as * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; and **** p ≤ 0.0001. Statistical comparison among groups was performed by mean +/- SEM using one-way ANOVA followed by Tukey multiple comparison test (n = 5 mice/group). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2076-2607/10/9/1871), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IL-17/IL-17A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:5000

Western Blot

1:1000
Application Notes
This purified antibody has been tested for use in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kDa. By western blot from a reducing gel expect a band approximately 15.0 kDa in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-17/IL-17A

Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

UniProt

Q61453 (Rat)

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-17/IL-17A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-17/IL-17A Antibody - Azide and BSA Free

Customer Reviews for IL-17/IL-17A Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review IL-17/IL-17A Antibody - Azide and BSA Free and earn rewards!

Have you used IL-17/IL-17A Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for IL-17/IL-17A Antibody - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies