IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.
IL-1ra/IL-1F3/IL1RN Antibody (694204) [PE]
Novus Biologicals | Catalog # FAB4801P
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Rat IgG1 Clone # 694204
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IL-1ra/IL-1F3
Arg8-Gln159
Accession # Q542W1
Arg8-Gln159
Accession # Q542W1
Specificity
Detects mouse IL-1ra/IL-1F3 in direct ELISAs and Western blots. In Western blots, approximately 30%-50% cross-reactivity with recombinant equine IL-1ra and recombinant human (rh) IL-1ra is observed. Less than 5% cross-reactivity with recombinant mouse (rm) IL-1b, rmIL-18, rmIL-36Ra, rmIL-36a, rmIL-36b, rmIL-1F10, rhIL-36, rhIL-1F7, or recombinant porcine IL-1ra, or recombinant rat IL-1ra is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Applications for IL-1ra/IL-1F3/IL1RN Antibody (694204) [PE]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
Additional IL-1ra/IL-1F3 Products
Product Documents for IL-1ra/IL-1F3/IL1RN Antibody (694204) [PE]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-1ra/IL-1F3/IL1RN Antibody (694204) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
