IL-2 R beta Antibody (TU27) - Low Endotoxin

Novus Biologicals | Catalog # NBP3-09104

Clone TU27 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TU27

Format

Low Endotoxin
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Product Specifications

Immunogen

TL-Mor cell line

Specificity

The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images for IL-2 R beta Antibody (TU27) - Low Endotoxin

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Separation of human CD122 positive CD56 positive CD3 negative NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml) GAM APC.
Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Surface staining pattern of human peripheral whole blood stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml) GAM APC.
Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104]

Flow Cytometry: IL-2 R beta Antibody (TU27) - Low Endotoxin, Azide and BSA Free [NBP3-09104] - Multicolor surface staining of human CD3 negative lymphocytes stained using anti-human CD122 (TU27) purified antibody (concentration in sample 4 ug/ml, GAM APC) and anti-human CD56 (LT56) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).

Applications for IL-2 R beta Antibody (TU27) - Low Endotoxin

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-2 R beta

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Gene Symbol

IL2RB

Additional IL-2 R beta Products

Product Documents for IL-2 R beta Antibody (TU27) - Low Endotoxin

Certificate of Analysis

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Product Specific Notices for IL-2 R beta Antibody (TU27) - Low Endotoxin

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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