IL-21R Antibody (DMC225) - Chimeric

Name: IL-21R Antibody (DMC225) - Chimeric
Brand: Novus Biologicals
SKU: NBP3-48299
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48299

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # DMC225

View all IL-21R Primary Antibodies »

Product Specifications

Immunogen

IL-21R recombinant protein expressed in mammalian cells (Accession# Q9HBE5). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for IL-21R Antibody (DMC225) - Chimeric

Flow Cytometry: IL-21R Antibody (DMC225) - Chimeric [NBP3-48299]

Flow cytometry analysis of antigen binding of anti-human IL21R mAb(NBP3-48299). (A) NBP3-48299 does not bind to 293T cells that do not express IL21R. (B) A clear peak shift of NBP3-48299 was seen compared to the control when incubated with IL21R-expressing Raji cells, indicating strong binding of NBP3-48299 to IL21R. Antibodies were incubated at 5 ug/mL.

Flow Cytometry: IL-21R Antibody (DMC225) - Chimeric [NBP3-48299]

Flow cytometry analysis with Anti-IL-21R (DMC225) on HEK293 cells transfected with human IL-21R (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Applications for IL-21R Antibody (DMC225) - Chimeric

Application

Recommended Usage

ELISA

1:5000-10000

Flow Cytometry

1:100

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

IL21 Receptor is a novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Long Name

Interleukin 21 Receptor

Alternate Names

CD360, IL-21 R, IL21R, NILR

Gene Symbol

IL21R

Additional IL-21R Products

Product Documents for IL-21R Antibody (DMC225) - Chimeric

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL-21R Antibody (DMC225) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.