IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.
Rat IL-23 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-06781
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 ul)
Sensitivity
9.38 pg/mL
Assay Range
15.63 - 1000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat IL-23 in samples. No significant cross-reactivity or interference between Rat IL-23 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) 5.5
Inter-Assay Precision (Precision between assays) 4.6
Recovery for Rat IL-23 ELISA Kit (Colorimetric)
Recovery
87 - 108 %
Linearity
Scientific Data Images for Rat IL-23 ELISA Kit (Colorimetric)
ELISA: Rat IL-23 ELISA Kit (Colorimetric) [NBP3-06781] -
ELISA: Rat IL-23 ELISA Kit (Colorimetric) [NBP3-06781] - Standard Curve ReferenceKit Contents for Rat IL-23 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: IL-23
Long Name
Interleukin 23
Alternate Names
IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF
Gene Symbol
IL23A
Additional IL-23 Products
Product Documents for Rat IL-23 ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rat IL-23 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
