Recombinant Porcine IL-3 Protein

Novus Biologicals | Catalog # NBP3-11061

Novus Biologicals
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Key Product Details

Applications

Western Blot, ELISA
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Product Specifications

Description

Recombinant Pig Interleukin-3 (IL-3) is a recombinant protein corresponding to aa 24-144 of native pig IL-3.

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Porcine IL-3 Protein

ELISA: Recombinant Porcine IL-3 Protein [NBP3-11061]

ELISA: Recombinant Porcine IL-3 Protein [NBP3-11061]

ELISA: Recombinant Porcine IL-3 Protein [NBP3-11061] - Recombinant Pig Interleukin-3 detected using Rabbit anti Pig Interleukin-3 as the capture reagent and Rabbit anti Pig Interleukin-3:Biotin as the detection reagent followed by Streptavidin:HRP.

Formulation, Preparation, and Storage

NBP3-11061
Preparation Method Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography.
Formulation PBS
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute with 20 uL sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3

IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells. Recombinant human IL-3 is a 17 kDa globular protein consisting of 133 amino acid residues.

Long Name

Interleukin 3

Alternate Names

IL3, MCGF, MULTI-CSF

Gene Symbol

IL3

Additional IL-3 Products

Product Documents for Recombinant Porcine IL-3 Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Porcine IL-3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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