Human IL-33 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-84818

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 uL)

Sensitivity

0.2 pg/mL

Assay Range

7.8 - 500 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The assay detects both natural and recombinant human IL-33. The cross-reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-33 positive sample. No cross reactivity or interference was detected, notably not with IL-1 alpha, IL-1 beta, and IL-18.

Description

The human IL-33 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-33

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.7%

Inter-Assay Precision (Precision between assays) CV% 6.9%

Recovery for Human IL-33 ELISA Kit (Colorimetric)

Recovery

99%

Scientific Data Images for Human IL-33 ELISA Kit (Colorimetric)

Sandwich ELISA: IL-33 ELISA Kit (Colorimetric) [NBP1-84818] - Representative standard curve for human IL-33 ELISA. Human IL-33 was diluted in serial 2-fold steps in CalibratorDiluent. Do not use this standard curve to derive test results. A standard curve must be run for each group ofmicrowell strips assayed.

Kit Contents for Human IL-33 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal polyclonal antibody to human IL-33
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-33 polyclonal polyclonal antibody
  • Calibrator Diluent
  • Conjugate Diluent
  • Human IL-33 Standard lyophilized, 500 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-33

IL-33 is a member of the IL-1 family of cytokines, which includes IL-1alpha, IL-1 beta, IL-18 and IL-1Ra. In humans, IL-33 mRNA is predominantly found in dermal fibroblasts, bronchial and small airway epithelial cells and smooth muscle cells of skin and lung tissues. This cytokine is localized primarily in the nucleus where it is thought to play a role in transcriptional repression but can also be released from the cell. Upon wounding or infection, IL-33 is released from the cell via an atypical secretory pathway. During apoptosis, IL-33 is cleaved by caspases-3 and 7 (and most likely not caspase-1) to produce an 18-kDa fragment which is released. This cleaved form of IL-33 can bind the ST2 receptor, without subsequent signaling events. Necrosis-mediated release of IL-33 enables binding to ST2, a member of the IL-1 receptor family found on mast and Th2 effector cells, leading to the release of Th2 cytokines. The cytokine production due to IL-33 in turn activates human basophils. IL-33 influence on activation, degranulation, enhanced adhesion and survival of eosinophils has been shown. IL-33 also increases synthesis of IL-6, IL-13, IL-1beta, TNF-alpha, prostaglandin D2 and MCP-1 by primary bone marrow-derived mast cells. Other cells activated by IL-33 are cardiomyocytes, glial cells and CD34+ cells.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Gene Symbol

IL33

Additional IL-33 Products

Product Documents for Human IL-33 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human IL-33 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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