Human IL-33 Quantikine ELISA Kit

D3300B 23 Citations Datasheet / COA / SDS

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QC244: Quantikine Immunoassay Control Group 244 for Human IL-33

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Citations (23)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-33 Quantikine ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (10 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

1.51 pg/mL

Assay Range

3.1 - 200 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-33.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-33 Immunoassay is a 4.0 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision over one lot of kit components. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	31	59.8	119	31	59.5	117
Standard Deviation	1.15	2.72	6.99	1.35	2.6	6.98
CV%	3.7	4.5	5.9	4.4	4.4	6

Recovery

The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	97	88-107
Cell Lysis Buffer (n=4)	103	86-123
EDTA Plasma (n=4)	91	83-103
Extraction Buffer (1X) (n=2)	103	81-115
Heparin Plasma (n=4)	89	85-95
Serum (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Data Examples

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Human IL-33 ELISA Standard Curve

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Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve

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Human IL-33 ELISA Serum/Plasma Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-33

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

Long Name:

Interleukin 33

Entrez Gene IDs:

90865 (Human); 77125 (Mouse)

Alternate Names:

C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-33 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
Authors: E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra
Journal of Translational Medicine, 2021;19(1):517.
Species: Human
Sample Types: Vitreous Humor
Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
Authors: II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie
Journal of Clinical Medicine, 2021;10(18):.
Species: Human
Sample Types: Tissue Homogenates
Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis
Authors: A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
Nature Communications, 2021;12(1):5947.
Species: Human
Sample Types: Cell Culture Supernates
Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability?
Authors: H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa
Clinical neurology and neurosurgery, 2021;206(0):106700.
Species: Human
Sample Types: Plasma
Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry
Authors: T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga
Allergol Int, 2020;0(0):.
Species: Human
Sample Types: Serum
COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
J. Immunol., 2020;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience
Authors: YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li
BMC Cardiovasc Disord, 2020;20(1):170.
Species: Human
Sample Types: Plasma
Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
Authors: P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek
Mediators Inflamm., 2020;2020(0):8465083.
Species: Human
Sample Types: Serum
Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
Cytokine, 2019;120(0):258-263.
Species: Human
Sample Types: Serum
Serum IL-1 Î² can be a biomarker in children with severe persistent allergic rhinitis
Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
Allergy Asthma Clin Immunol, 2019;15(0):58.
Species: Human
Sample Types: Serum

Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions
Authors: AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab
BMC Pulm Med, 2019;19(1):114.
Species: Human
Sample Types: Serum

Serum sST2 levels predict severe exacerbation of asthma
Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
Respir. Res., 2018;19(1):169.
Species: Human
Sample Types: Serum

Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis
Authors: E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette
Arthritis Res. Ther., 2018;20(1):122.
Species: Human
Sample Types: Serum

In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity
Authors: M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku
Cell. Mol. Immunol., 2018;0(0):.
Species: Human
Sample Types: Serum

Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Plasma

Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris
Authors: RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli
Mediators Inflamm., 2017;2017(0):7151285.
Species: Human
Sample Types: Serum

Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
Sci Rep, 2017;7(1):16760.
Species: Human
Sample Types: Cell Culture Supernates

Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis
Authors: JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park
BMC Pulm Med, 2017;17(1):39.
Species: Human
Sample Types: BALF

Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy
Authors: MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry
J Neuroinflammation, 2017;14(1):58.
Species: Human
Sample Types: Plasma

Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
Sci Rep, 2016;6(0):32116.
Species: Human
Sample Types: Plasma

IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
J Transl Med, 2015;13(0):381.
Species: Human
Sample Types: Serum

High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue.
Authors: Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D
Cytokine, 2013;61(3):766-71.
Species: Human
Sample Types: Plasma

The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma.
Authors: Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I
Clin Exp Allergy, 0;46(6):884-7.
Species: Human
Sample Types: Serum