Human IL-33 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 31 | 59.8 | 119 | 31 | 59.5 | 117 |
Standard Deviation | 1.15 | 2.72 | 6.99 | 1.35 | 2.6 | 6.98 |
CV% | 3.7 | 4.5 | 5.9 | 4.4 | 4.4 | 6 |
Recovery
The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 97 | 88-107 |
Cell Lysis Buffer (n=4) | 103 | 86-123 |
EDTA Plasma (n=4) | 91 | 83-103 |
Extraction Buffer (1X) (n=2) | 103 | 81-115 |
Heparin Plasma (n=4) | 89 | 85-95 |
Serum (n=4) | 89 | 85-96 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: IL-33
IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.
Citations for Human IL-33 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
23
Citations: Showing 1 - 10
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Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
Authors: E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra
Journal of Translational Medicine, 2021;19(1):517.
Species: Human
Sample Types: Vitreous Humor
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Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
Authors: II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie
Journal of Clinical Medicine, 2021;10(18):.
Species: Human
Sample Types: Tissue Homogenates
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Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis
Authors: A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
Nature Communications, 2021;12(1):5947.
Species: Human
Sample Types: Cell Culture Supernates
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Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability?
Authors: H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa
Clinical neurology and neurosurgery, 2021;206(0):106700.
Species: Human
Sample Types: Plasma
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Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry
Authors: T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga
Allergol Int, 2020;0(0):.
Species: Human
Sample Types: Serum
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COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
J. Immunol., 2020;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience
Authors: YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li
BMC Cardiovasc Disord, 2020;20(1):170.
Species: Human
Sample Types: Plasma
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Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
Authors: P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek
Mediators Inflamm., 2020;2020(0):8465083.
Species: Human
Sample Types: Serum
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Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
Cytokine, 2019;120(0):258-263.
Species: Human
Sample Types: Serum
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Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
Allergy Asthma Clin Immunol, 2019;15(0):58.
Species: Human
Sample Types: Serum
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Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions
Authors: AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab
BMC Pulm Med, 2019;19(1):114.
Species: Human
Sample Types: Serum
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Serum sST2 levels predict severe exacerbation of asthma
Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
Respir. Res., 2018;19(1):169.
Species: Human
Sample Types: Serum
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Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis
Authors: E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette
Arthritis Res. Ther., 2018;20(1):122.
Species: Human
Sample Types: Serum
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In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity
Authors: M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku
Cell. Mol. Immunol., 2018;0(0):.
Species: Human
Sample Types: Serum
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Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
Cytokine, 2018;0(0):.
Species: Human
Sample Types: Plasma
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Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris
Authors: RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli
Mediators Inflamm., 2017;2017(0):7151285.
Species: Human
Sample Types: Serum
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Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
Sci Rep, 2017;7(1):16760.
Species: Human
Sample Types: Cell Culture Supernates
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Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis
Authors: JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park
BMC Pulm Med, 2017;17(1):39.
Species: Human
Sample Types: BALF
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Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy
Authors: MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry
J Neuroinflammation, 2017;14(1):58.
Species: Human
Sample Types: Plasma
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Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
Sci Rep, 2016;6(0):32116.
Species: Human
Sample Types: Plasma
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IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
J Transl Med, 2015;13(0):381.
Species: Human
Sample Types: Serum
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High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue.
Authors: Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D
Cytokine, 2013;61(3):766-71.
Species: Human
Sample Types: Plasma
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The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma.
Authors: Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I
Clin Exp Allergy, 0;46(6):884-7.
Species: Human
Sample Types: Serum
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