Human IL-33 Quantikine ELISA Kit

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Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve
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Product Details
Citations (31)
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Human IL-33 Quantikine ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (10 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
1.51 pg/mL
Assay Range
3.1 - 200 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant human IL-33.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-33 Immunoassay is a 4.0 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.


The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-107
Cell Lysis Buffer (n=4) 103 86-123
EDTA Plasma (n=4) 91 83-103
Extraction Buffer (1X) (n=2) 103 81-115
Heparin Plasma (n=4) 89 85-95
Serum (n=4) 89 85-96


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-33 ELISA Linearity
Human IL-33 ELISA Linearity

Scientific Data

Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve

Human IL-33 ELISA Standard Curve

Human IL-33 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-33

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

Long Name:
Interleukin 33
Entrez Gene IDs:
90865 (Human); 77125 (Mouse)
Alternate Names:
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Citations for Human IL-33 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Endothelial CD34 expression and regulation of immune cell response in-vitro
    Authors: Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Airway epithelium IgE-Fc?RI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma
    Authors: Weng, CM;Lee, MJ;Chao, W;Lin, YR;Chou, CJ;Chen, MC;Chou, CL;Lin Tsai, I;Lin, CH;Fan Chung, K;Kuo, HP;
    Mucosal immunology
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea
    Authors: P Szczepanik, M Michalska-, M Kowal, D Krasowska
    Journal of Clinical Medicine, 2022-10-28;11(21):.
    Species: Human
    Sample Types: Serum
  4. Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
    Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
    Nature Immunology, 2022-07-06;23(7):1021-1030.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma
    Authors: K Rasheed, U Moens, B Policastro, JI Johnsen, V Koljonen, H Sihto, WO Lui, B Sveinbjørn
    International Journal of Molecular Sciences, 2022-03-28;23(7):.
    Species: Human
    Sample Types: Plasma
  6. Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
    Authors: E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra
    Journal of Translational Medicine, 2021-12-20;19(1):517.
    Species: Human
    Sample Types: Vitreous Humor
  7. Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer
    Authors: X Zhang, K Bi, X Tu, Q Zhang, Q Cao, Y Liang, P Zeng, L Wang, T Liu, W Fang, H Diao
    Cancer Medicine, 2021-10-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis
    Authors: A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Nature Communications, 2021-10-12;12(1):5947.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates, Serum, Tissue Homogenates
  9. Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
    Authors: II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie
    Journal of Clinical Medicine, 2021-09-12;10(18):.
    Species: Human
    Sample Types: Tissue Homogenates
  10. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021-07-20;36(3):109392.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability?
    Authors: H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa
    Clinical neurology and neurosurgery, 2021-05-20;206(0):106700.
    Species: Human
    Sample Types: Plasma
  12. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
    Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
    J. Immunol., 2020-07-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry
    Authors: T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga
    Allergol Int, 2020-07-08;0(0):.
    Species: Human
    Sample Types: Serum
  14. IL-33 and its decoy sST2 in patients with Alzheimer's disease and mild cognitive impairment
    Authors: M Saresella, I Marventano, F Piancone, F La Rosa, D Galimberti, C Fenoglio, E Scarpini, M Clerici
    J Neuroinflammation, 2020-06-06;17(1):174.
    Species: Human
    Sample Types: CSF
  15. High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience
    Authors: YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li
    BMC Cardiovasc Disord, 2020-04-15;20(1):170.
    Species: Human
    Sample Types: Plasma
  16. Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
    Authors: P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek
    Mediators Inflamm., 2020-04-15;2020(0):8465083.
    Species: Human
    Sample Types: Serum
  17. Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
    Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
    Allergy Asthma Clin Immunol, 2019-09-18;15(0):58.
    Species: Human
    Sample Types: Serum
  18. Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions
    Authors: AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab
    BMC Pulm Med, 2019-06-25;19(1):114.
    Species: Human
    Sample Types: Serum
  19. Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
    Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
    Cytokine, 2019-05-29;120(0):258-263.
    Species: Human
    Sample Types: Serum
  20. Serum sST2 levels predict severe exacerbation of asthma
    Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
    Respir. Res., 2018-09-03;19(1):169.
    Species: Human
    Sample Types: Serum
  21. In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity
    Authors: M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku
    Cell. Mol. Immunol., 2018-07-20;0(0):.
    Species: Human
    Sample Types: Serum
  22. Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis
    Authors: E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette
    Arthritis Res. Ther., 2018-06-08;20(1):122.
    Species: Human
    Sample Types: Serum
  23. Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
    Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
    Cytokine, 2018-04-12;0(0):.
    Species: Human
    Sample Types: Plasma
  24. Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
    Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
    Sci Rep, 2017-12-01;7(1):16760.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy
    Authors: MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry
    J Neuroinflammation, 2017-03-20;14(1):58.
    Species: Human
    Sample Types: Plasma
  26. Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris
    Authors: RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli
    Mediators Inflamm., 2017-02-22;2017(0):7151285.
    Species: Human
    Sample Types: Serum
  27. Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis
    Authors: JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park
    BMC Pulm Med, 2017-02-15;17(1):39.
    Species: Human
    Sample Types: BALF
  28. Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
    Sci Rep, 2016-08-24;6(0):32116.
    Species: Human
    Sample Types: Plasma
  29. The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma.
    Authors: Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I
    Clin Exp Allergy, 2016-06-01;46(6):884-7.
    Species: Human
    Sample Types: Serum
  30. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015-12-16;13(0):381.
    Species: Human
    Sample Types: Serum
  31. High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue.
    Authors: Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D
    Cytokine, 2013-01-26;61(3):766-71.
    Species: Human
    Sample Types: Plasma


  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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