Interleukin 36 beta (IL-36 beta), also known as IL-1F8, FIL-1h and IL-1H2, is a member of the IL-1 family of proteins. IL-36 beta is reported to be actively secreted. Cells expressing IL-36 beta include resting and activated monocytes, and B cells. The receptor for IL-36 beta is thought to be a combination of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta activates pathways involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner.
IL-36 beta/IL-1F8 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-83892
![Immunohistochemistry-Paraffin: IL-36 beta/IL-1F8 Antibody [NBP1-83892]](https://resources.rndsystems.com/images/products/IL-36-beta-IL-1F8-Antibody-Immunohistochemistry-Paraffin-NBP1-83892-img0001.jpg "Immunohistochemistry-Paraffin: IL-36 beta/IL-1F8 Antibody [NBP1-83892]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PKSYAIRDSRQMVWVLSGNSLIAAPLSRSIKPVTLHLIACRDTEFSDKEKGNMVYLGIKGKDLCLFCAEIQGKP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IL-36 beta/IL-1F8 Antibody - BSA Free
Immunohistochemistry-Paraffin: IL-36 beta/IL-1F8 Antibody [NBP1-83892]
Immunohistochemistry-Paraffin: IL-36 beta/IL-1F8 Antibody [NBP1-83892] - Staining of human placenta shows distinct membranous and cytoplasmic positivity in decidua cells.![IL-36 beta/IL-1F8 Antibody - BSA Free Western Blot: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP1-83892]](https://resources.rndsystems.com/images/products/nbp1-83892_rabbit-polyclonal-il-36-beta-il-1f8-antibody-84202516574417.jpg "Western Blot: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP1-83892]")
Western Blot: IL-36 beta/IL-1F8 Antibody - BSA Free [NBP1-83892]
Analysis in control (vector only transfected HEK293T lysate) and IL36B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Applications for IL-36 beta/IL-1F8 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IL-36 beta/IL-1F8
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Alternate Names
FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B
Gene Symbol
IL36B
Additional IL-36 beta/IL-1F8 Products
Product Documents for IL-36 beta/IL-1F8 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-36 beta/IL-1F8 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for IL-36 beta/IL-1F8 Antibody - BSA Free
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Q: We recently purchased a vial of IL1F8 Antibody (NBP1-83892), lot R32798. The concentration is not listed, and I was wondering if you could provide me with that information?
A: The concentration for Lot: R32798 is 0.1mg/ml.
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Associated Pathways
