Human IL-38/IL-1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-38 shares 82% aa sequence identity with mouse IL-1F10. Human IL-38 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.
IL-38/IL-1F10 Antibody [DyLight 405]
Novus Biologicals | Catalog # AF2427E
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human IL‑38/IL‑1F10
Cys2-Trp152
Accession # Q8WWZ1
Cys2-Trp152
Accession # Q8WWZ1
Specificity
Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1 F9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for IL-38/IL-1F10 Antibody [DyLight 405]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: IL-38/IL-1F10
Long Name
Interleukin 1 Family Member 10
Alternate Names
FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38
Gene Symbol
IL1F10
Additional IL-38/IL-1F10 Products
Product Documents for IL-38/IL-1F10 Antibody [DyLight 405]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for IL-38/IL-1F10 Antibody [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
