Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Immunocytochemistry, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-38/IL-1F10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL‑38/IL‑1F10
Cys2-Trp152
Accession # Q8WWZ1

Specificity

Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL-1ra and rhIL-1F5 is observed and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL-18, rhIL-1F6, rhIL-1F7, rhIL-1F8, and rhIL-1 F9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL‑38/IL‑1F10 Antibody

Human IL-38/IL-1F10 Antibody in ELISA Standard Curve.

Human IL‑38/IL‑1F10 ELISA Standard Curve.

Recombinant Human IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-38/IL-1F10 Monoclonal Antibody(Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-38/IL-1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IL‑38/IL‑1F10 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑38/IL‑1F10 Monoclonal Antibody(Catalog # MAB24271).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-38/IL-1F10 DuoSet ELISA Kit (Catalog # DY9110-05) for convenient development of a sandwich ELISA.

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑38/IL‑1F10

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-38/IL-1F10

Human IL‑38/IL‑1F10 is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 R1. Human IL-1F10 shares 82% aa sequence identity with mouse IL-1F10. Human IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra and IL-1 beta, respectively.

Long Name

Interleukin 1 Family Member 10

Alternate Names

FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38

Entrez Gene IDs

84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)

Gene Symbol

IL1F10

UniProt

Q8WWZ1

Additional IL-38/IL-1F10 Products

Product Documents for Human IL‑38/IL‑1F10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL‑38/IL‑1F10 Antibody

For research use only

Related Research Areas

IL-1 Family

Citations for Human IL‑38/IL‑1F10 Antibody

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FAQs

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail