IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-75193

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Inhibition Activity

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # rhPM-1 (Tocilizumab)

Format

Azide and BSA Free
View all IL6R Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by CDR grafting of mouse PM-1 antibody onto human REI and NEW framework regions to create rhPM-1.

Specificity

Tocilizumab binds specifically to both the membrane bound and soluble forms of human IL-6R. The IL6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

Immunofluorescence: IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free [NBP2-75193]

Immunofluorescence: IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free [NBP2-75193]

Immunofluorescence: IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric [NBP2-75193] - Immunofluorescence staining of fixed U937 cells with anti-IL-6 receptorantibody rhPM-1 (Tocilizumab). Immunofluorescence analysis of paraformaldehyde fixed U937 cells on coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of rhPM-1 (Tocilizumab) (NBP2-75193) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75193, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: IL6R

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Alternate Names

CD126, CD126 antigen, gp80, IL-6 receptor subunit alpha, IL-6R 1, IL-6R subunit alpha, IL-6R-1, IL6RA, IL-6RA, IL-6R-alpha, interleukin 6 receptor, interleukin 6 receptor alpha subunit, interleukin-6 receptor subunit alpha, Membrane glycoprotein 80, MGC104991, biosimilar antibody, biosimilars

Gene Symbol

IL6R

Additional IL6R Products

Product Documents for IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL6R Antibody (rhPM-1 (Tocilizumab)) - Chimeric - Azide and BSA Free

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Protocols

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FAQs

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