ILF3 Antibody

Novus Biologicals | Catalog # NBP1-71876

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 325 and 375 of human Nuclear Factor of Activated T-cells 90 kDa, 110 kDa using the numbering given in entry NP_036350.2 (GeneID 3609).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILF3 Antibody

Western Blot: ILF3 Antibody [NBP1-71876]

Western Blot: ILF3 Antibody [NBP1-71876]

Western Blot: ILF3 Antibody [NBP1-71876] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NF90/NF110 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NF90 and/or NF110 were also immunoprecipitated by rabbit anti-NF90/NF110 antibodies NBP1-71874 and NBP1-71875 as well as anti-NF110 antibody NBP1-71877, each of which recognizes a different epitope.
Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-71876]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-71876]

Immunohistochemistry-Paraffin: ILF3 Antibody [NBP1-71876] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- NF90/NF110 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for ILF3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 -1:1000

Immunohistochemistry-Paraffin

1:100 -1:1000

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ILF3

ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

Alternate Names

Double-stranded RNA-binding protein 76, double-stranded RNA-binding protein, 76 kD, DRBF, DRBP76M-phase phosphoprotein 4, dsRNA binding protein NFAR-2/MPP4, interleukin enhancer binding factor 3, 90kDa, interleukin enhancer-binding factor 3, MPHOSPH4interleukin enhancer binding factor 3, 90kD, MPP4MMP4, NF90CBTF, NFAR-1, NFAR2, NFARNF110, NF-AT-90, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, nuclear factor of activated T-cells, 90 kD, TCP110, TCP80, Translational control protein 80

Entrez Gene IDs

3609 (Human)

Gene Symbol

ILF3

UniProt

Q12906 (Human)

Additional ILF3 Products

Product Documents for ILF3 Antibody

Certificate of Analysis

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Product Specific Notices for ILF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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