ILK Antibody (6A5) - BSA Free

Novus Biologicals | Catalog # NBP3-26198

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6A5 expressed in HEK293

Format

BSA Free
View all ILK Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ILK [UniProt Q13418]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ILK Antibody (6A5) - BSA Free

ILK Antibody (6A5)

Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] -

Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] - Immunoprecipitating ILK in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26198 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26198 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
ILK Antibody (6A5)

Western Blot: ILK Antibody (6A5) [NBP3-26198] -

Western Blot: ILK Antibody (6A5) [NBP3-26198] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: ILK Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 52, 45, 37 kDa
Observed band size: 51 kDa
ILK Antibody (6A5)

Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] -

Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] - Staining of MCF7 Cells with ILK Antibody (6A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for ILK Antibody (6A5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ILK

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Gene Symbol

ILK

Additional ILK Products

Product Documents for ILK Antibody (6A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for ILK Antibody (6A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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