ILK Antibody

Novus Biologicals | Catalog # NB100-809

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ILK Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DMIVPILEKMQDK corresponding to C-Terminus according to NP_004508.1, NP_001014794.1, NP_001014795.1.

Specificity

All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat ILK Antibody (NB100-809) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ILK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ILK Antibody

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ILK Antibody [NB100-809]

Immunohistochemistry-Paraffin: ILK Antibody [NB100-809]

Immunohistochemistry-Paraffin: ILK Antibody [NB100-809] - (4ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809]

Western Blot: ILK Antibody [NB100-809] - (0.03ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ILK Antibody

Application
Recommended Usage

Immunohistochemistry

2-4ug/ml

Immunohistochemistry-Paraffin

2-4ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01-0.03ug/ml
Application Notes
Western blot: Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysate (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
IHC: In paraffin embedded Human Kidney shows preferential staining of PCT.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ILK

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Entrez Gene IDs

3611 (Human); 11846 (Mouse); 29221 (Rat)

Gene Symbol

ILK

UniProt

NP_004508.1 (Human)

Additional ILK Products

Product Documents for ILK Antibody

Certificate of Analysis

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Product Specific Notices for ILK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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