IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Novus Biologicals | Catalog # NBP3-16786

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4X3S10 expressed in HEK293
View all IMP Dehydrogenase 2/IMPDH2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMP Dehydrogenase 2/IMPDH2 (P12268). QANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of lysates from wild type(WT) and IMP Dehydrogenase 2/IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C6 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of NIH/3T3 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -

Immunocytochemistry/ Immunofluorescence: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Confocal imaging of C2C12 cells using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] -

Western Blot: IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10) [NBP3-16786] - Western blot analysis of various lysates, using [KO Validated] IMP Dehydrogenase 2/IMPDH2 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Applications for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IMP Dehydrogenase 2/IMPDH2

IMPDH2 encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

Long Name

Inosine 5'-Monophosphate Dehydrogenase 2

Alternate Names

IMDH2, IMP Oxireductase 2, IMPD2, IMPDH2

Gene Symbol

IMPDH2

Additional IMP Dehydrogenase 2/IMPDH2 Products

Product Documents for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

Certificate of Analysis

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Product Specific Notices for IMP Dehydrogenase 2/IMPDH2 Antibody (4X3S10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

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Protocols

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