Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00003836-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A4-1B5

Format

Azide and BSA Free
View all Importin alpha 5/KPNA1 Primary Antibodies »

Product Specifications

Immunogen

KPNA1 (AAH02374.1, 1 a.a. ~ 538 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQINNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL

Reactivity Notes

Rat reactivity reported in customer review.

Specificity

KPNA1 - karyopherin alpha 1 (importin alpha 5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Analysis of KPNA1 expression in transfected 293T cell line by KPNA1 monoclonal antibody (M01), clone 2A4-1B5.Lane 1: KPNA1 transfected lysate(60.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Immunohistochemistry-Paraffin: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Immunohistochemistry-Paraffin: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Analysis of monoclonal antibody to KPNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.
Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

Western Blot: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - KPNA1 monoclonal antibody (M01), clone 2A4-1B5 Analysis of KPNA1 expression in HeLa.
ELISA: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

ELISA: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01]

ELISA: Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) [H00003836-M01] - Detection limit for recombinant GST tagged KPNA1 is approximately 1ng/ml as a capture antibody.

Applications for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IHC-P and ELISA. ICC/IF usage reported in scientific literature (PMID: 21741962). Use in immunoprecipitation reported in scientific literature (PMID: 26052702).

Reviewed Applications

Read 1 review rated 5 using H00003836-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Importin alpha 5/KPNA1

Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination

Alternate Names

IPOA5, KPNA1, NPI-1, RCH2, SRP1

Entrez Gene IDs

3836 (Human)

Gene Symbol

KPNA1

OMIM

600686 (Human)

UniProt

AAH02374 (Human)

Additional Importin alpha 5/KPNA1 Products

Product Documents for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free

Customer Reviews for Importin alpha 5/KPNA1/SRP1 Antibody (2A4-1B5) - Azide and BSA Free (1)

5 out of 5
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    Application: Western Blot
    Sample Tested: Human intestinal epithelial cell (Caco2), Sample Amount: 40 ug
    Species: Human
    Verified Customer | Posted 07/01/2009

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