Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87703

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Indoleamine 2,3-dioxygenase/IDO Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-IDO1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining in human placenta and cerebral cortex tissues. Corresponding IDO1 RNA-seq data are presented for the same tissues.
Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Analysis in control (vector only transfected HEK293T lysate) and IDO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human placenta shows moderate to strong cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human lymph node shows strong positivity in non - germinal center cells.
Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703]

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87703] - Staining of human small intestine shows moderate to strong positivity in lymphoid cells.

Applications for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Indoleamine 2,3-dioxygenase/IDO

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.42). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine.[supplied by OMIM]

Alternate Names

3dioxygenase, IDO, IDO1, INDO, Indoleamine 2

Gene Symbol

IDO1

Additional Indoleamine 2,3-dioxygenase/IDO Products

Product Documents for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Indoleamine 2,3-dioxygenase/IDO Antibody - BSA Free

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Protocols

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FAQs

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