Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

Novus Biologicals | Catalog # NB100-2073

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Influenza A Virus H1N1

Cited:

Human, Frog - Xenopus (African Clawed Frog), Virus

Applications

Validated:

Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 14C2
View all Influenza A H1N1 M2 Primary Antibodies »

Product Specifications

Immunogen

M2 protein from A/WSN/33-infected CV1 cell lysate.

Specificity

Influenza A H1N1 M2 (A/WSN/1933)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933) [NB100-2073]

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933) [NB100-2073]

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933) [NB100-2073] - Analysis of MDCK cells

Applications for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

10 ug/ml

Western Blot

1 ug/ml
Application Notes
Blocking usage reported in literature (PMID: 7571410). WB: Detects an approx. 15 kDa protein representing the M2 protein. It has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain. May be useful in IHC.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 M2

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Alternate Names

Influenza M2

Additional Influenza A H1N1 M2 Products

Product Documents for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

Certificate of Analysis

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Product Specific Notices for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

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Protocols

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FAQs for Influenza A H1N1 M2 Antibody (14C2) - (A/WSN/1933)

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  • Q: Could you cite this antibody's purity % as well as the means by which purity was determined?

    A: This particular antibody was protein G purified from ascites. We unfortunately do not measure the purity of our antibodies, but it is expected to be above 95%.

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