Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Novus Biologicals | Catalog # NBP2-14997

Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Influenza A H1N1 M2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to Influenza A H1N1 M2 (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Specificity

Influenza A H1N1 M2 (A/Puerto Rico/8/1934)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997]

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997]

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997] - Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A H1N1 M2 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37CC, 30 min.
Western Blot: Influenza A H1N1 M2 Antibody(A/Puerto Rico/8/1934) [NBP2-14997]

Western Blot: Influenza A H1N1 M2 Antibody(A/Puerto Rico/8/1934) [NBP2-14997]

Western Blot: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997] - Sample (50 ug of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE; antibody diluted at 1:5000.
Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Western Blot: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997] -

Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (NBP2-14997) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 M2

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Alternate Names

Influenza M2

UniProt

H1N1 (Human)

Additional Influenza A H1N1 M2 Products

Product Documents for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

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Protocols

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